AHL Diagnostic Section: Molecular

Avian Influenza (AI) PCR – Bovine

Avian Influenza (AI) PCR – Bovine
Samples must be submitted with the AIV Submission Form (linked below under Specimen Requirements). Mark the outside of the box with Molecular or AIV to alert staff of proper unpackaging.
SPECIES
Bovine
DIAGNOSTIC SECTION
Molecular
SETUP DAY
Submissions must be received by Wednesday, 12:00pm sample receipt cutoff.
SPECIMEN AMOUNT AND TYPE
>6mL raw milk in a 15mL or 50mL screw-top conical tube, no more than 75% full; no snap cap containers.
SHIPPING REQUIREMENTS
Store and ship milk samples cold, not frozen, on indirect ice packs. Each sample should be in an individual, sealable bag with absorbent material, paperwork in a separate sealed bag. Package in leak-proof container with absorbent material.
PRICE
N/A
SPECIMEN REQUIREMENTS

AIV Laboratory Sample Submission Form

No preservatives added. Samples must be no more than 5 days old. 

TIME REQUIRED
2-5 business days
COLLECTION PROTOCOL

Veterinarian collected and submitted samples only. Click here for the ISDA AIV Sample Collection Guide.

ADDITIONAL INFORMATION

All samples also tested for AIV ELISA per federal guidance. Please note, all non-negative results will require forwarding to NVSL for confirmation. Final report will not be issued until NVSL results are received by ISDA.

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Avian Influenza (AI) PCR – Avian

Avian Influenza (AI) PCR – Avian
Samples must be submitted with the AIV Submission Form (linked below under Specimen Requirements). Mark the outside of the box with Molecular or AIV to alert staff of proper unpackaging.
SPECIES
Avian
DIAGNOSTIC SECTION
Molecular
SETUP DAY
Upon request
SPECIMEN AMOUNT AND TYPE
Tracheal, Oropharyngeal, or Cloacal Swab(s)
SHIPPING REQUIREMENTS
Store and ship swabs cold, not frozen, on indirect ice packs. Package in leak-proof container with absorbent material
PRICE
N/A
SPECIMEN REQUIREMENTS

AIV Laboratory Sample Submission Form

BHI or MTM Transport Media only. Samples must be no more than 3 days old.

TIME REQUIRED
2-5 business days
COLLECTION PROTOCOL

Veterinarian collected and submitted samples only. Click here for the ISDA AIV Sample Collection Guide.

ADDITIONAL INFORMATION

All swabs tested automatically for subtypes H5/H7 or Newcastle’s Disease. Actual additional testing is dependent on initial results. Please note, all non-negative results will require forwarding to NVSL for confirmation. Final report will not be issued until NVSL results are received by ISDA.

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Tritrichomonas foetus Pooled PCR

Tritrichomonas foetus Pooled PCR
LAB WILL POOL. If pool is positive - additional charge of $26 (tube) / $32 (pouch) for each sample to test individually.
SPECIES
Bovine
DIAGNOSTIC SECTION
Molecular
SETUP DAY
Upon request
SPECIMEN AMOUNT AND TYPE
Send samples inoculated into any of the following: BioMed InPouch; BioMed TF-Transit tube; 10 mL screw-top tube with 3-5 mL PBS; 15 mL centrifuge tube with 3-5 mL PBS. LAB WILL POOL
SHIPPING REQUIREMENTS
Freeze samples after collection. Ship with frozen gel pack.
PRICE
Tube $42.00 each pool, Pouch $57.00 each pool. Sample pools (up to 5 samples) containing PCR inhibitors may require additional testing and incur additional charges - see additional information below.
SPECIMEN REQUIREMENTS

Samples for PCR testing do not require incubation. Freeze samples after collection.

LAB WILL POOL

TIME REQUIRED
2-5 days Turnaround times may be delayed due to seasonal factors.
COLLECTION PROTOCOL

Bulls: collect smegma from glans penis/swab prepuce sheath

Cows: collect cervical mucus or vaginal discharge

ADDITIONAL INFORMATION

If inhibitors are present, a dilution and retest can be run for $20/pool. If the dilution and retest is still showing signs of inhibition, a clarification reextraction and retest can be run for $45/pool. If inhibition remains, all samples from the pool(s) will be resulted as indeterminate.

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Tritrichomonas foetus PCR

Tritrichomonas foetus PCR
SPECIES
Bovine
DIAGNOSTIC SECTION
Molecular
SETUP DAY
Upon request
SPECIMEN AMOUNT AND TYPE
Send samples inoculated into any of the following: BioMed InPouch; BioMed TF-Transit tube; 10 mL screw-top tube with 3-5 mL PBS; 15 mL centrifuge tube with 3-5 mL PBS.
SHIPPING REQUIREMENTS
Freeze samples after collection. Ship with frozen gel pack.
PRICE
Tube $26.00, Pouch $32.00. Samples containing PCR inhibitors may require additional testing and incur additional charges - see additional information below.
SPECIMEN REQUIREMENTS

Samples for PCR testing do not require incubation. Freeze samples after collection.

TIME REQUIRED
2-5 days Turnaround times may be delayed due to seasonal factors.
COLLECTION PROTOCOL

Bulls: collect smegma from glans penis/swab prepuce sheath

Cows: collect cervical mucus or vaginal discharge

ADDITIONAL INFORMATION

If inhibitors are present, a dilution and retest can be run for $20. If the dilution and retest is still showing signs of inhibition, a clarification reextraction and retest can be run for $33. If inhibition remains, the sample(s) will be resulted as indeterminate.

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Johne’s (paratuberculosis) PCR-Pooled

Johne’s (paratuberculosis) PCR-Pooled
Up to 5 samples per pool LAB WILL POOL
SPECIES
Bovine
DIAGNOSTIC SECTION
Molecular
SETUP DAY
Upon request
SPECIMEN AMOUNT AND TYPE
>5 g Feces (send individual samples)
SHIPPING REQUIREMENTS
Ship samples cold or frozen. Package in leak-proof container with absorbent material.
PRICE
$53.00 per pool
SPECIMEN REQUIREMENTS

Feces

LAB WILL POOL

TIME REQUIRED
3-5 days
COLLECTION PROTOCOL

Collect fresh feces. Do not cross contaminate with other samples.

ADDITIONAL INFORMATION

N/A

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Johne’s (paratuberculosis) PCR-Individual

Johne’s (paratuberculosis) PCR-Individual
SPECIES
Bovine
DIAGNOSTIC SECTION
Molecular
SETUP DAY
Upon request
SPECIMEN AMOUNT AND TYPE
>5 g Feces
SHIPPING REQUIREMENTS
Ship samples cold or frozen. Package in leak-proof container with absorbent material.
PRICE
$46.50
SPECIMEN REQUIREMENTS

Feces

TIME REQUIRED
3-5 days
COLLECTION PROTOCOL

Collect fresh feces. Do not cross contaminate with other samples.

ADDITIONAL INFORMATION

N/A

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Bovine Viral Diarrhea (BVD) PCR-Pooled

Bovine Viral Diarrhea (BVD) PCR-Pooled
SPECIES
Bovine
DIAGNOSTIC SECTION
Molecular
SETUP DAY
Upon request
SPECIMEN AMOUNT AND TYPE
1 cm Ear Notch (with no saline/preservatives)
SHIPPING REQUIREMENTS
Ship as soon as possible (overnight recommended) or freeze immediately (avoid freeze/thaw). Ship samples cold on indirect ice packs. Package in leak-proof container with absorbent material.
PRICE
$162.00
SPECIMEN REQUIREMENTS

Ear Notch (1 cm with no saline/preservatives)

LAB WILL POOL (up to 24 samples per pool)

TIME REQUIRED
3-5 days
COLLECTION PROTOCOL

Aseptic collection. Place each sample in a sterile individual sealed container.

ADDITIONAL INFORMATION

N/A

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Bovine Viral Diarrhea (BVD) PCR-Individual

Bovine Viral Diarrhea (BVD) PCR-Individual
SPECIES
Bovine
DIAGNOSTIC SECTION
Molecular
SETUP DAY
Upon request
SPECIMEN AMOUNT AND TYPE
1 cm Ear Notch (with no saline/preservatives)
SHIPPING REQUIREMENTS
Ship as soon as possible (overnight recommended) or freeze immediately (avoid freeze/thaw). Ship samples cold on indirect ice packs. Package in leak-proof container with absorbent material.
PRICE
$42.00
SPECIMEN REQUIREMENTS

Ear Notch (1 cm with no saline/preservatives)

TIME REQUIRED
3-5 days
COLLECTION PROTOCOL

Aseptic collection. Place sample in individual sealed container.

ADDITIONAL INFORMATION

N/A

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